It but green light is used allowed on

It is
an instrument in which very small and minute objects are seen by the help of
microscope is known as microscopy .Microscope is an instrument in which  image of an object is clearly observed.

TYPES OF MICROSCOPY

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1.            Light microscopy (COMPOUND MICROSCOPE)

§  Bright
field microscopy

§  Dark
field microscopy

2.         Polarization microscopy

3.       Confocal microscopy

4.       Electron microscopy

§  Transmission
Electron Microscope (TEM)

§  Scanning
Electron microscope

            
5.             Fluorescence
microscopy

            
6.             Immunofluorescence
microscopy

 

FLUORESCENCE MICROSCOPY

In
this type of microscopy Fluorescent materials emit visible light when they are
illuminated with ultraviolet (UV) rays.

§  PRINCIPLE:

 Mercury arc lamp of high intensity is used as
the light source. White light is emitted; which passed through an ‘exciter
filter’. Blue component is allowed of the white light (the white light consists
of seven colors, order of wavelength is violet, indigo, blue, green, yellow,
orange and red) decreasing the seven colours to pass through so all other color
components are blocked.

 

§  RAY DIAGRAM

A mirror, blue light is reflected, but
green light is used allowed on the way of the blue light. Such an angle the
mirror is fixed that the blue light is reflected downward to the specimen.

§  FLUORESCENSE SPECTRA:

It is a range of spectra of fluorescence particles  in which strokes shift are defined in terms
of excitation and emission of particles.

§  REQUIREMENT

Required sample should be fixed and living .

APPLICATION:

§  In laboratories, fluorescence microscopy involve in
diagnosis of tuberclosis.

§  Fluorescence 
antibodies are involved in immunospecific disease.

§  Involved to identify pathogenicity of bacteria

§  By the helpof this technique we can measure Ph and acidy
of compartmentalized organ .

§  Involve in calcium imaging .

§  Identifies localized sepration of organs

§  Measures signaling network of cells

§  ADVANTAGES:

§  It  seems living
cells

§  Can give 100 times more clearith than other microscopy

DISADVANTAGES:

§  Very high cost

§  Complex instrument

 

 

IMMUNOFLUORESCENCE
MICROSCOPY

Antibodies are used by microscope labeled with a
fluorescing substance and
fluorescence microscope to detect the binding of the antibody through the
production of a characteristic visible light under UV light.

 It is
an instrument in which very small and minute objects are seen by the help of
microscope is known as microscopy .Microscope is an instrument in which  image of an object is clearly observed.

TYPES OF MICROSCOPY

1.            Light microscopy (COMPOUND MICROSCOPE)

§  Bright
field microscopy

§  Dark
field microscopy

2.         Polarization microscopy

3.       Confocal microscopy

4.       Electron microscopy

§  Transmission
Electron Microscope (TEM)

§  Scanning
Electron microscope

            
5.             Fluorescence
microscopy

            
6.             Immunofluorescence
microscopy

 

FLUORESCENCE MICROSCOPY

In
this type of microscopy Fluorescent materials emit visible light when they are
illuminated with ultraviolet (UV) rays.

§  PRINCIPLE:

 Mercury arc lamp of high intensity is used as
the light source. White light is emitted; which passed through an ‘exciter
filter’. Blue component is allowed of the white light (the white light consists
of seven colors, order of wavelength is violet, indigo, blue, green, yellow,
orange and red) decreasing the seven colours to pass through so all other color
components are blocked.

 

§  RAY DIAGRAM

A mirror, blue light is reflected, but
green light is used allowed on the way of the blue light. Such an angle the
mirror is fixed that the blue light is reflected downward to the specimen.

§  FLUORESCENSE SPECTRA:

It is a range of spectra of fluorescence particles  in which strokes shift are defined in terms
of excitation and emission of particles.

§  REQUIREMENT

Required sample should be fixed and living .

APPLICATION:

§  In laboratories, fluorescence microscopy involve in
diagnosis of tuberclosis.

§  Fluorescence 
antibodies are involved in immunospecific disease.

§  Involved to identify pathogenicity of bacteria

§  By the helpof this technique we can measure Ph and acidy
of compartmentalized organ .

§  Involve in calcium imaging .

§  Identifies localized sepration of organs

§  Measures signaling network of cells

§  ADVANTAGES:

§  It  seems living
cells

§  Can give 100 times more clearith than other microscopy

DISADVANTAGES:

§  Very high cost

§  Complex instrument

 

 

IMMUNOFLUORESCENCE
MICROSCOPY

Antibodies are used by microscope labeled with a
fluorescing substance and
fluorescence microscope to detect the binding of the antibody through the
production of a characteristic visible light under UV light.

 It is
an instrument in which very small and minute objects are seen by the help of
microscope is known as microscopy .Microscope is an instrument in which  image of an object is clearly observed.

TYPES OF MICROSCOPY

1.            Light microscopy (COMPOUND MICROSCOPE)

§  Bright
field microscopy

§  Dark
field microscopy

2.         Polarization microscopy

3.       Confocal microscopy

4.       Electron microscopy

§  Transmission
Electron Microscope (TEM)

§  Scanning
Electron microscope

            
5.             Fluorescence
microscopy

            
6.             Immunofluorescence
microscopy

 

FLUORESCENCE MICROSCOPY

In
this type of microscopy Fluorescent materials emit visible light when they are
illuminated with ultraviolet (UV) rays.

§  PRINCIPLE:

 Mercury arc lamp of high intensity is used as
the light source. White light is emitted; which passed through an ‘exciter
filter’. Blue component is allowed of the white light (the white light consists
of seven colors, order of wavelength is violet, indigo, blue, green, yellow,
orange and red) decreasing the seven colours to pass through so all other color
components are blocked.

 

§  RAY DIAGRAM

A mirror, blue light is reflected, but
green light is used allowed on the way of the blue light. Such an angle the
mirror is fixed that the blue light is reflected downward to the specimen.

§  FLUORESCENSE SPECTRA:

It is a range of spectra of fluorescence particles  in which strokes shift are defined in terms
of excitation and emission of particles.

§  REQUIREMENT

Required sample should be fixed and living .

APPLICATION:

§  In laboratories, fluorescence microscopy involve in
diagnosis of tuberclosis.

§  Fluorescence 
antibodies are involved in immunospecific disease.

§  Involved to identify pathogenicity of bacteria

§  By the helpof this technique we can measure Ph and acidy
of compartmentalized organ .

§  Involve in calcium imaging .

§  Identifies localized sepration of organs

§  Measures signaling network of cells

§  ADVANTAGES:

§  It  seems living
cells

§  Can give 100 times more clearith than other microscopy

DISADVANTAGES:

§  Very high cost

§  Complex instrument

 

 

IMMUNOFLUORESCENCE
MICROSCOPY

Antibodies are used by microscope labeled with a
fluorescing substance and
fluorescence microscope to detect the binding of the antibody through the
production of a characteristic visible light under UV light.